Evaluating and improving cDNA sequence quality with cQC

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Evaluating and improving cDNA sequence quality with cQC

SUMMARY Errors are prevalent in cDNA sequences but the extent to which sequence collections differ in frequencies and types of errors has not been investigated systematically. cDNA quality control, or cQC, was developed to evaluate the quality of cDNA sequence collections and to revise those sequences that differ from a higher quality genomic sequence. After removing rRNA, vector, bacterial ins...

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ESTprep: Preprocessing CDNA Sequence Reads

MOTIVATION High accuracy of data always governs the large-scale gene discovery projects. The data should not only be trustworthy but should be correctly annotated for various features it contains. Sequence errors are inherent in single-pass sequences such as ESTs obtained from automated sequencing. These errors further complicate the automated identification of EST-related sequencing. A tool is...

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The cDNA and Derived Amino Acid Sequence

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ژورنال

عنوان ژورنال: Bioinformatics

سال: 2005

ISSN: 1460-2059,1367-4803

DOI: 10.1093/bioinformatics/bti709